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periostin  (Novus Biologicals)


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    Novus Biologicals periostin
    (A) <t>Periostin</t> ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.
    Periostin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+postn+antibody/Periostin%2FOSF-2+Antibody+-+C-terminus+-+Azide+and+BSA+Free/bio_rxiv__64898__2026__02__17__706476-60-14-15
    Average 94 stars, based on 36 article reviews
    periostin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state"

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state

    Journal: bioRxiv

    doi: 10.64898/2026.02.17.706476

    (A) Periostin ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.
    Figure Legend Snippet: (A) Periostin ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.

    Techniques Used: Expressing, Western Blot

    Related Articles

    Staining:

    Article Title: The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium
    Article Snippet: .. For the detection of periostin (POSTN), sections from DSPP Cerulean /DMP1 Cherry animals were stained with anti‐POSTN antibody (Novus Biologicals, Littleton, CO, USA; NBP1‐30042) diluted 1:200 overnight at 4°C. .. Subsequently, the sections were stained with donkey anti‐rabbit secondary antibody conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Waltham, MA, USA; A31573) diluted 1:500 at room temperature for 2 hours.



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    (A) <t>Periostin</t> ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.
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    Image Search Results


    Intercellular communication between POSTN + CAFs and APOE + TAMs in LSCC. ( A ) Global ligand–receptor interaction networks reveal strong bidirectional communication between POSTN + CAFs and APOE + TAMs. ( B ) Outgoing signaling patterns from POSTN + CAFs. ( C ) Outgoing signaling patterns from APOE + TAMs. (In figure A-C, the numbers on the edges indicate the amount of inferred significant intercellular interactions between cell populations.) ( D ) Bubble plots highlight significant ligand–receptor pairs mediating communication from POSTN + CAFs. ( E ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from POSTN + CAFs to APOE + TAMs). ( F ) KEGG analysis identifies cytokine-cytokine receptor interactions, complement/coagulation as dominant pathways (from POSTN + CAFs to APOE + TAMs). ( G ) Bubble plots highlight significant ligand–receptor pairs mediating communication from APOE + TAMs. ( H ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from APOE + TAMs to POSTN + CAFs). ( I ) KEGG analysis identifies proteoglycans in cancer, cytokine-cytokine receptor interaction as dominant pathways (from APOE + TAMs to POSTN + CAFs).

    Journal: International Journal of General Medicine

    Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

    doi: 10.2147/IJGM.S583478

    Figure Lengend Snippet: Intercellular communication between POSTN + CAFs and APOE + TAMs in LSCC. ( A ) Global ligand–receptor interaction networks reveal strong bidirectional communication between POSTN + CAFs and APOE + TAMs. ( B ) Outgoing signaling patterns from POSTN + CAFs. ( C ) Outgoing signaling patterns from APOE + TAMs. (In figure A-C, the numbers on the edges indicate the amount of inferred significant intercellular interactions between cell populations.) ( D ) Bubble plots highlight significant ligand–receptor pairs mediating communication from POSTN + CAFs. ( E ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from POSTN + CAFs to APOE + TAMs). ( F ) KEGG analysis identifies cytokine-cytokine receptor interactions, complement/coagulation as dominant pathways (from POSTN + CAFs to APOE + TAMs). ( G ) Bubble plots highlight significant ligand–receptor pairs mediating communication from APOE + TAMs. ( H ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from APOE + TAMs to POSTN + CAFs). ( I ) KEGG analysis identifies proteoglycans in cancer, cytokine-cytokine receptor interaction as dominant pathways (from APOE + TAMs to POSTN + CAFs).

    Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

    Techniques: Chemotaxis Assay, Coagulation

    Correlation and spatial colocalization between POSTN + CAFs and APOE + TAMs in LSCC. ( A – C ) Scatter plots showing the positive correlation between POSTN + CAF abundance and APOE + TAM abundance across multiple cohorts, including TCGA-HNSC, GSE41613 , and GSE65858 . Shaded areas represent 95% confidence intervals, and the correlation was evaluated using Spearman’s test. ( D ) Representative multiplex immunofluorescence staining images of LSCC tissues showing colocalization of POSTN (red) and APOE (green) proteins. DAPI (blue) marks cell nuclei.

    Journal: International Journal of General Medicine

    Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

    doi: 10.2147/IJGM.S583478

    Figure Lengend Snippet: Correlation and spatial colocalization between POSTN + CAFs and APOE + TAMs in LSCC. ( A – C ) Scatter plots showing the positive correlation between POSTN + CAF abundance and APOE + TAM abundance across multiple cohorts, including TCGA-HNSC, GSE41613 , and GSE65858 . Shaded areas represent 95% confidence intervals, and the correlation was evaluated using Spearman’s test. ( D ) Representative multiplex immunofluorescence staining images of LSCC tissues showing colocalization of POSTN (red) and APOE (green) proteins. DAPI (blue) marks cell nuclei.

    Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

    Techniques: Multiplex Assay, Immunofluorescence, Staining

    Protein–protein interaction between POSTN and APOE. ( A ) Structural model of the POSTN-APOE complex; the inset highlights key hydrogen-bonding and hydrophobic interactions. ( B and C ) Electrostatic potential maps of POSTN ( B ) and APOE ( C ), showing charge complementarity at the interface. ( D ) A 2D interaction diagram illustrating hydrogen bonds and hydrophobic contacts between key residues. Symbols used: red lines for hydrogen bonds, blue circles for non-ligand bonds, and pink arcs for hydrophobic contacts.

    Journal: International Journal of General Medicine

    Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

    doi: 10.2147/IJGM.S583478

    Figure Lengend Snippet: Protein–protein interaction between POSTN and APOE. ( A ) Structural model of the POSTN-APOE complex; the inset highlights key hydrogen-bonding and hydrophobic interactions. ( B and C ) Electrostatic potential maps of POSTN ( B ) and APOE ( C ), showing charge complementarity at the interface. ( D ) A 2D interaction diagram illustrating hydrogen bonds and hydrophobic contacts between key residues. Symbols used: red lines for hydrogen bonds, blue circles for non-ligand bonds, and pink arcs for hydrophobic contacts.

    Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

    Techniques:

    Prognostic and functional implications of POSTN + CAFs and APOE + TAMs co-enrichment. ( A ) Kaplan-Meier survival analysis shows the worst outcomes in the POSTN + CAFs high /APOE + TAMs high group. ( B ) GO analysis reveals enrichment in extracellular matrix organization and collagen regulation in the POSTN + CAFs high /APOE + TAMs high group. ( C ) GSEA indicates activation of EMT, antigen presentation, neuroactive ligand–receptor signaling, and TGF-β pathways in the POSTN + CAFs high /APOE + TAMs high group.

    Journal: International Journal of General Medicine

    Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

    doi: 10.2147/IJGM.S583478

    Figure Lengend Snippet: Prognostic and functional implications of POSTN + CAFs and APOE + TAMs co-enrichment. ( A ) Kaplan-Meier survival analysis shows the worst outcomes in the POSTN + CAFs high /APOE + TAMs high group. ( B ) GO analysis reveals enrichment in extracellular matrix organization and collagen regulation in the POSTN + CAFs high /APOE + TAMs high group. ( C ) GSEA indicates activation of EMT, antigen presentation, neuroactive ligand–receptor signaling, and TGF-β pathways in the POSTN + CAFs high /APOE + TAMs high group.

    Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

    Techniques: Functional Assay, Activation Assay, Immunopeptidomics

    Association of the POSTN + CAFs/APOE + TAMs axis with immune suppression and ICI resistance. ( A ) TME immune cell infiltration across LSCC clinical subgroups quantified by five deconvolution algorithms. ( B ) High POSTN + CAFs/APOE + TAMs enrichment LSCC shows increased stromal and immune scores but reduced tumor purity, as well as TIDE scores with a tendency to be higher. ( C – E ) The IMvigor210 cohort validates poorer survival in POSTN + CAFs high /APOE + TAMs high patients. ( F ) Treatment-response comparison shows a higher proportion of PD/SD in the high-risk group ( χ² -test).

    Journal: International Journal of General Medicine

    Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

    doi: 10.2147/IJGM.S583478

    Figure Lengend Snippet: Association of the POSTN + CAFs/APOE + TAMs axis with immune suppression and ICI resistance. ( A ) TME immune cell infiltration across LSCC clinical subgroups quantified by five deconvolution algorithms. ( B ) High POSTN + CAFs/APOE + TAMs enrichment LSCC shows increased stromal and immune scores but reduced tumor purity, as well as TIDE scores with a tendency to be higher. ( C – E ) The IMvigor210 cohort validates poorer survival in POSTN + CAFs high /APOE + TAMs high patients. ( F ) Treatment-response comparison shows a higher proportion of PD/SD in the high-risk group ( χ² -test).

    Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

    Techniques: Comparison

    Subpopulation distribution of hub genes. (A) Clustering visualization of subpopulations in the corpus cavernosum. (B) Expression distribution of hub genes in different cells of corpus cavernosum. The expression distribution of POSTN (C, D) and LOX (E, F) in the tissue of corpus cavernosum. UMAP: uniform manifold approximation and projection, EC: endothelial cell, FB: fibroblast, SMC: smooth muscle cell, SWC: Schwann cell, MAC: macrophage, T: T cell.

    Journal: The World Journal of Men's Health

    Article Title: Comprehensive Analysis of N6-Methyladenosine Modification Profiling in Diabetic Erectile Dysfunction

    doi: 10.5534/wjmh.240328

    Figure Lengend Snippet: Subpopulation distribution of hub genes. (A) Clustering visualization of subpopulations in the corpus cavernosum. (B) Expression distribution of hub genes in different cells of corpus cavernosum. The expression distribution of POSTN (C, D) and LOX (E, F) in the tissue of corpus cavernosum. UMAP: uniform manifold approximation and projection, EC: endothelial cell, FB: fibroblast, SMC: smooth muscle cell, SWC: Schwann cell, MAC: macrophage, T: T cell.

    Article Snippet: Similarly, the sections of corpus cavernosum were subjected to immunohistochemistry to detect the protein expression of POSTN (66491-1-Ig, Proteintech) and LOX (A11504, ABclonal).

    Techniques: Expressing

    (A) Periostin ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.

    Journal: bioRxiv

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state

    doi: 10.64898/2026.02.17.706476

    Figure Lengend Snippet: (A) Periostin ( Postn ) expression assessed by qPCR in primary cardiac fibroblasts treated for 72 h. (B-C) Representative Western blot (B) and densitometry normalized to GAPDH and Ctrl (C) of Periostin across 6 biological replicates. (D-E) Representative Western blots for α-SMA (D) and densitometric quantification (E) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (F-G) Representative Western blots for periosotin (F) and densitometric quantification (G) following treatment with p38 MAPK inhibitor SB203580 (10 μM). (H) Total soluble collagen secretion measured by Picrosirius Red. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA or two-way ANOVA as appropriate.

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies (1:1,000 in 5% BSA): Periostin (Novus Biologicals, NBP1-30042), α-SMA (Cell Signaling, D4K9N), Col1a1 (Cell Signaling, E8F47), Fibronectin (Cell Signaling, E7F5X), and GAPDH (Cell Signaling, 14C10).

    Techniques: Expressing, Western Blot